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ICES Journal of Marine Science: Journal du Conseil Advance Access published online on April 23, 2009
ICES Journal of Marine Science: Journal du Conseil, doi:10.1093/icesjms/fsp110
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© 2009 International Council for the Exploration of the Sea. Published by Oxford Journals. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Molecular identification of European flounder (Platichthys flesus) and its hybrids with European plaice (Pleuronectes platessa)

Agnieszka Kijewska, Artur Burzynski and Roman Wenne

Department of Genetics and Marine Biotechnology, Polish Academy of Sciences, Institute of Oceanology, Powstanców Warszawy 55, 81-712 Sopot, Poland

Correspondence to R. Wenne: tel: +48 58 731 17 63; fax: +48 58 731 18 63; e-mail: rwenne{at}iopan.gda.pl.

Kijewska, A., Burzynski, A., and Wenne, R. 2009. Molecular identification of European flounder (Platichthys flesus) and its hybrids with European plaice (Pleuronectes platessa). – ICES Journal of Marine Science, 66: 000–000.

European flounder (Platichthys flesus) and plaice (Pleuronectes platessa) are commercially important marine fish species inhabiting the continental shelf waters of Europe. Morphological similarity between the two makes it difficult to identify their hybrids, so species misclassification can generate errors in defining stocks in terms of their conservation and management. Flounder and plaice populations from the North Sea and the Baltic Sea were studied. Mitochondrial DNA (mtDNA) was used to confirm the morphological species identification. The set of molecular markers, two mitochondrial (cytochrome b and D-loop) and two nuclear (the ribosomal marker ITS and parathyroid hormone-related protein gene), was constructed to identify the two flatfish species and their hybrids. "Pure" flounder (P. flesus) were observed in the Bay of Gdansk, Baltic Sea, and off the coast of Denmark in the North Sea. The fishing area near Bornholm in the Baltic is rich in P. flesus x P. platessa hybrids. The length difference of the amplified D-loop fragment was used for species identification. The characteristics of heteroplasmy in the control region (D-loop) can be useful as a population marker in the European flounder. Our studies demonstrate the utility of mtDNA polymorphism combined with nuclear molecular markers for correct identification of the morphologically similar and hybridized European flounder and plaice.

Keywords: hybridization, mitochondrial DNA, nuclear markers, Platichthys flesus, Pleuronectes platessa

Received 31 October 2008; accepted 29 March 2009.


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