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ICES Journal of Marine Science: Journal du Conseil Advance Access originally published online on January 16, 2009
ICES Journal of Marine Science: Journal du Conseil 2009 66(3):561-565; doi:10.1093/icesjms/fsn218
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© 2009 International Council for the Exploration of the Sea. Published by Oxford Journals. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

A simple genetic identification method for Northeast Atlantic smoothhound sharks (Mustelus spp.)

Edward D. Farrell1, Maurice W. Clarke2 and Stefano Mariani1

1 Marine Biodiversity, Ecology and Evolution, School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland
2 Marine Institute, Oranmore, Galway, Ireland

Correspondence to S. Mariani: tel: +353 1 716 2347; fax: +353 1 716 1153; e-mail: stefano.mariani{at}ucd.ie.

Farrell, E. D., Clarke, M. W., and Mariani, S. 2009. A simple genetic identification method for Northeast Atlantic smoothhound sharks (Mustelus spp.). – ICES Journal of Marine Science, 66: 561–565.

Considerable ambiguity exists in the identification of the commercially valuable smoothhound sharks (Mustelus spp.) in the Northeast (NE) Atlantic. The lack of a clear and accurate method of identification prevents the collation of reliable species-specific landings and survey data for these fish and hinders the accurate delineation of the distribution ranges of species and stock boundaries, making it impossible to apply sound species-specific conservation and management strategies. This paper reports on the development of a multiplex PCR reaction that utilizes a set of mtDNA primers for the identification of Mustelus asterias, Mustelus mustelus, and Galeorhinus galeus. The high throughput method allows for the rapid and cost-effective identification of large numbers of samples; its application to 431 fish collected between 2006 and 2008 also raises important questions regarding the biogeography of the genus Mustelus in the NE Atlantic.

Keywords: conservation, fisheries management, genetic identification method, mitochondrial DNA, multiplex PCR, Mustelus, ND2, sharks

Received 13 October 2008; accepted 16 December 2008; advance access publication 16 January 2009.


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