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ICES Journal of Marine Science: Journal du Conseil Advance Access originally published online on November 28, 2006
ICES Journal of Marine Science: Journal du Conseil 2007 64(2):328-331; doi:10.1093/icesjms/fsl023
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© 2006 International Council for the Exploration of the Sea. Published by Oxford Journals. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of Girella punctata and G. leonina by PCR-RFLP analysis

Shiro Itoi, Takashi Saito, Mai Shimojo, Sayaka Washio and Haruo Sugita

Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-8510, Japan

Correspondence to H. Sugita: tel/fax: +81 466 84 3679; e-mail: sugita{at}brs.nihon-u.ac.jp

Itoi, S., Saito, T., Shimojo, M., Washio, S., and Sugita, H. 2007. Identification of Girella punctata and G. leonina by PCR-RFLP analysis. – ICES Journal of Marine Science, 64: 328–331.

Two Girella species, Girella punctata and G. leonina, are sympatric sister species with an extensive overlap in their respective distributions on shallow rocky reefs from Hong Kong to the south of the Japanese Islands. Juveniles of the two species cannot be discriminated easily on the basis of external characters. In this study, after morphological identification of the species, sequencing analysis was carried out for the partial 16S ribosomal RNA gene and for the D-loop region in mitochondrial DNA. A total of 109 specimens was examined. Restriction site mapping of the sequences suggested that the electrophoretic patterns of polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) analysis of the gene products would produce a species-specific banding pattern. Subsequently, the PCR-RFLP analysis showed that the method was as effective for separating the two morphologically similar species of the genus Girella as the sequencing analysis.

Keywords: D-loop, Girella leonina, Girella punctata, mtDNA, PCR–RFLP, 16S rRNA

Received 3 February 2006; accepted 5 October 2006; advance access publication 28 November 2006.


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