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ICES Journal of Marine Science: Journal du Conseil 2005 62(8):1683-1690; doi:10.1016/j.icesjms.2005.05.011
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© 2005 International Council for the Exploration of the Sea

A double DNA approach for identifying Macrorhamphosus scolopax (Pisces, Centriscidae)

Nikoletta Karaiskou, Alexander Triantafyllidis, Maritsa Margaroni, Dimitris Karatzas and Costas Triantaphyllidis*

Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki 54 124 Thessaloniki, Macedonia, Greece

*Correspondence to C. Triantaphyllidis: tel: +30 231 0998309; fax: +30 231 0998374. e-mail: triant{at}bio.auth.gr.

DNA-based methodologies are increasingly used successfully to elucidate cases of misidentification of adult individuals of fish species with morphological similarities. However, the problem of misidentification can arise even earlier than adulthood, between congeneric and even distantly related species with similar morphology in the early developmental stages. Therefore, a double DNA approach was developed for Macrorhamphosus scolopax, to identify and discriminate it from European species of the genus Trachurus. These species overlap geographically and temporally and are characterized by morphologically similar eggs. The approach looks at both mitochondrial and nuclear loci. Polymerase Chain Reaction (PCR) amplification of the 16S rRNA mtDNA gene was followed by restriction analysis with two species-specific enzymes: EcoRV and PmlI. Digestion with these endonucleases yielded species-specific electrophoretic profiles. Additionally, the nuclear multi-copy 5S rRNA gene was selected as an alternative candidate for identifying M. scolopax. The universality of the results was verified by screening a large number of fish from five geographical regions, covering most of the overlapping distribution of the species. The output is a double DNA methodology that can be used for egg identification and which could be of value in the egg production method of biomass assessment.

Keywords: genetic identification, M. scolopax, PCR–RFLP, 5S rRNA, 16S rRNA

Received 29 March 2005; accepted 26 May 2005.


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