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ICES Journal of Marine Science: Journal du Conseil 2004 61(5):864-867; doi:10.1016/j.icesjms.2004.03.003
© 2004 by ICES/CIEM International Council for the Exploration of the Sea/Conseil International pour l'Exploration de la Mer
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A mitochondrial DNA PCR–RFLP marker for population studies of the black scabbardfish (Aphanopus carbo)

Ricardo Quinta*, Laurentina Gomes and Ana Teia dos Santos

Instituto Nacional Investigação Agrária e das Pescas/Instituto de Investigação das Pescas e do Mar Av. de Brasilia, 1449-006 Lisboa, Portugal

*Correspondence to R. Quinta: tel: +351 21 3027025; fax: +351 21 3015948. e-mail: rquinta{at}ipimar.pt.

Black scabbardfish (Aphanopus carbo), a commercially valuable marine fish off Portugal and the Madeira Archipelago, was surveyed for mitochondrial DNA variation of part of the cytochrome b gene. In all, 51 fish from three Northeast Atlantic localities were examined using ten restriction enzymes. Overall nucleon diversity was 0.180. Genetic differentiation ({theta}=0.25) was significant; the Madeira Archipelago sample was distinguishable from samples from the other two localities. The approach should be useful for a more extensive study of black scabbardfish populations.

Keywords: Aphanopus carbo, PCR–RFLP, stock identification

Received 12 January 2004; accepted 20 March 2004.


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